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Registros recuperados: 97
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Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites ArchiMer
Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf
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Holstein white coat color and performance: phenotypic, genetic and environmental correlations Braz. J. Genet.
Becerril,Carlos M.; Wilcox,Charles J.; Guerrero,Victor M..
Correlations of measures of percentages of white coat color, five measures of production and two measures of reproduction were obtained from 4293 first lactation Holsteins from eight Florida dairy farms. Percentages of white coat color were analyzed as recorded and transformed by an extension of Box-Cox procedures. Statistical analyses were by derivative-free restricted maximum likelihood (DFREML) with an animal model. Phenotypic and genetic correlations of white percentage (not transformed) were with milk yield, 0.047 and 0.097; fat yield, 0.002 and 0.004; fat percentage, -0.047 and -0.090; protein yield, 0.024 and 0.048; protein percentage, -0.070 and -0.116; days open, -0.012 and -0.065; and calving interval, -0.007 and -0.029. Changes in magnitude of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Holstein; Phenotypic; Genetic; Environmental.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400009
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Hybridization and morphological differences between the two closely related oyster taxa Crassostrea angulata and C. gigas ArchiMer
Batista, Frederico; Fonseca, Vera; Ben-hamadou, Radhouan; Taris, Nicolas; Henriques, Maria; Boudry, Pierre.
Unlike in some crops, no large heterotic effects have been observed in most farmed animals. Interestingly, significant hybrid vigour for some traits has been reported in the Pacific oyster, Crassostrea gigas. Crossbreeding between closely related taxa can be seen as an efficient way to exploit the dominance component of the genetic variance of a trait. Factorial crosses between the Portuguese oyster C. angulata (from Sado estuary, Portugal) and C. gigas (from Seudre estuary, France) were done to evaluate the aquaculture potential of hybrids. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under usual farming conditions. The genetic confirmation of all progenies was done using mitochondrial and nuclear markers in order to detect...
Tipo: Text Palavras-chave: Hybridization; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3429.pdf
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Identification of sources of resistance in sorghum to Peronosclerospora sorghi Trop. Plant Pathol.
Barbosa,Flavia C. R; Casela,Carlos R; Pfenning,Ludwig H; Santos,Fredolino G.
The main objective of this work was to identify sources of resistance in sorghum (Sorghum bicolor) to Peronosclerospora sorghi, the causal agent of downy mildew, through the evaluation of 42 sorghum genotypes under natural infection in the field. Genotypes were planted in single row plots between two rows of the susceptible line SC283, planted 30 days before, to act as spreader rows, in two separate nurseries. The experimental design was a completely randomized block design with three replications. Sorghum genotypes CMSXS156, CMSXS157, CMSXS243, TxARG-1, 8902, 9902054, 9910032, 9910296, Tx430, QL-3, SC170-6-17, CMSXS762 and BR304 were classified as highly resistant in both nurseries. Among these, SC170-6-17 and 9910296 showed 0% systemic infection. Results...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Sorghum bicolor; Diseases; Genetic; Resistance; Downy mildew.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000500010
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Image analysis : a new method for estimating triploidy in commercial bivalves ArchiMer
Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri.
For determination of ploidy levels in bivalves, three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.)....
Tipo: Text Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf
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Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf
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Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (Poster) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Early-life history of oysters, like in most marine bivalves, is characterized by high fecundity and low survival rate. In hatcheries, which are ecoming more and more important for the production of juveniles for aquaculture production, rearing conditions are optimised (density, feeding, temperature...), and contrast with those observed in the wild. Additionally, slow growing larvae are culled by sieving.
Tipo: Text Palavras-chave: Crassostrea gigas; Juvenile; Genetic variability; Selection; Genetic; Hatcheries; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3444.pdf
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Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (slides) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many marine bivalves, are characterized by very high fecundity and low survival rate at early stages of life. Rearing conditions in hatcheries (i.e. density, feeding, temperature...) are different from those observed in the wild. Additionally, slow growing larvae are culled by sieving. As previous studies have shown that genetic variability exists for several early development traits, we aimed to assess if common hatchery rearing practices exert specific selective processes at the larval stage. In order to avoid possible bias and to increase the number of families studied, we used a mixed-family approach with subsequent parentage analysis using a recently developed set of three multiplexed microsatellite markers. We studied the variance in...
Tipo: Text Palavras-chave: Hatcheries; Selection; Genetic drift; Genetic; Crassotrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3483.pdf
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Induced triploidy in the European clam, Ruditapes decussatus (L.) and performance of triploid larvae ArchiMer
Gerard, Andre; Naciri, Yamama; Noiret, Christophe; Ledu, Christophe; Peignon, Jean-marie.
For the first time, effective treatments using cytochalasin B were developed to induce triploidy in the European clam, Ruditapes decussatus (L.). The percentage of triploid embrios was assessed by karyological or image analysis. Two treatments (O.5 or 1 mg of cytochasin B (CB per ml of dimethylsulfoxide in 1 l of sea water) were applied at different times after fertilization (10 to 25 mn), for two different periods (15 and 20 min). Best results were obtained for a CB concentration of 1 mg/l. When treatment was applied 15 min after fertilization for a 20 min period, 94% and 95% of triploid embryos were obtained in two repeated experiments. At metamorphosis, the treated larvae appeared to be no smaller than the control larvae in all experiments. Thowever, in...
Tipo: Text Palavras-chave: Genetic; Ruditapes decussatus; Clam; Induced triploidy.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3208.pdf
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Initialism as a mechanism of weed interference: can a crop plant be blinded? Planta Daninha
Vidal,R.A.; Trezzi,M.M.; Kozlowski,L.A.; Prates,M.V.B.; Cieslik,L.F.; Merotto Jr.,A..
Initialism is a new word proposed to indicate the "shade-avoidance syndrome". Plants detect the presence of neighbor plants very early in the growing season through changes in light quality. They modify the allocation of photosynthesis products privileging shoot growth over the roots. One of the hypotheses of the authors is that, when weed management is timely scheduled, a "blind" crop could be more productive because it would avoid an imbalance on the shoot:root ratio (S:R). Two strategies were developed to test this hypothesis: a) to use the classical Yoda's Law to screen several crops for insensitivity to S:R imbalance; b) to evaluate several growth regulators to control the plant responses to crowding. Experimental results confirm that both strategies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Initialism; Shade-avoidance syndrome; Genetic; Growth regulator.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300002
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Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas? ArchiMer
Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C..
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf
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Mapping QTL for resistance to summer mortality in the Pacific oyster Crassostrea gigas ArchiMer
Sauvage, Christopher; Heurtebise, Serge; De Koning, D.; Haley, Chris; Boudry, Pierre; Lapegue, Sylvie.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). Such selective breeding would strongly benefit from the identification of markers associated to the resistance to allow the development of markers assisted selection. Recently, a set of a new kinds of markers (SNPs) have been developed in the Pacific oyster. These markers were identified and characterized in several genes involved in the immune response and energetic pathways (Sauvage, 2007). In this context, the mapping of SNPs, of 16 new EST-SSR based markers and of...
Tipo: Text Palavras-chave: Mortality; Genetic; Crassostrea gigas; Pacific oyster; Mapping QTL.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3504.pdf
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Melhoramento genético. Infoteca-e
PINTO, A. C. de Q.; SOUZA, V. A. B. de; ROSSETTO, C. J.; FERREIRA, F. R.; COSTA, J. G. da.
Citogenética da mangueira; recursos genéticos: domesticação e classificação da espécie, distribuição fitogenética, raças e espécies, coleções e Bancos Ativo de Germoplasma, potencial e uso dos recursos genéticos e seus problemas; o mecanismo de reprodução, a biologia floral e os fatores ambientais, a expressão sexual, a polinização e a incompatibilidade, o vingamento e a retenção de frutos. a embrionia e a estenospermocarpia, objetivos do melhoramento, principais métodos e estratégias para o melhoramento, a introdução, avaliação e seleção de cultivares (progenitores), melhoramento da população, métodod da seleção recorrente, teste de progênie, método da hibridação, seleção clonal, policruzamento e seleção entre populações de meio-irmãos, melhoramento para...
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Genetic; Genética; Manga; Melhoramento; Mangoes; Plant breeding.
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/134330
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Melhoramento genetico de mandioca. Infoteca-e
FUKUDA, W. M. G..
Variedade de mandioca e um dos principais componentes para o desenvolvimento do sistema produtivo dessa cultura, alem de constituir uma tecnologia que nao implica em custos adicionais para o produtor. Apesar da mandioca apresentar um alto potencial produtivo, estimado por COCK (1974) em 90 t/ha/ano, a produtividade nacional esta em torno de 12 t/ha. Um dos fatores que tem contribuido para a baixa produtividade desse cultivo no Brasil, e o uso de variedades com baixo potencial produtivo. A demanda por novas variedades de mandioca vem crescendo constantemente, a medida que surgem novas alternativas de uso pelo produto, bem como a expansao de novas fronteiras agricolas para o plantio dessa cultura. Para atender essas demandas, e importante definir-se as...
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Genetic; Propagation; Propagacao; Biologie.; Biologia; Manihot Esculenta.; Cassava.; Breeding.
Ano: 1995 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/637374
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Melhoramento genetico no Brasil - realizacoes e perspectivas. Infoteca-e
QUEIROZ, M. A. de.
O presente capítulo, complementa o enfoque dado pelos autores mencionados, de modo a ampliar o conhecimento sobre a contribuição do melhoramento de plantas no Brasil.
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Melhoramento de planta; Genetic; Chain; Production; Agronegócio; Agricultura; Cadeia Produtiva; Genética; Meio Ambiente; Agribusiness; Agriculture; Environment; Plant breeding.
Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/134341
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Metodologia de conservação de pólen de cana-de-açúcar. Infoteca-e
AMARAL, A. L. do; SANTOS, J. M. dos; CAMARA, T. M. M.; BARBOSA, G. V. de S..
bitstream/item/79630/1/Cot-1271.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Genética de planta; Melhoramento genético; Genetic; Sugar cane.; Cana-de-açúcar; Pólen.; Pollen..
Ano: 2012 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/953861
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MicroRNAs in veterinary cardiology Ciência Rural
Wolf,Marcela; Muehlbauer,Eloísa; Sousa,Marlos Gonçalves.
ABSTRACT: The use of biomarkers is an important recent development in veterinary medicine. Biomarkers allow non-invasive quantification of substances with diagnostic and prognostic potential in several diseases. The microRNAs are small, non-coding RNAs that regulate gene expression and are expressed in different forms in many diseases. Reduced or over-expression of microRNAs showed to be part of the pathogenesis of some heart diseases in humans and animals. Diagnostic and therapeutic value of measuring microRNAs in veterinary cardiology is increased because abnormal expression can be managed by the use of antagonists (in the case of overexpression) and mimicking (in the case of underexpression). Thus, this literature review aimed to compile scientific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Genetic; Biomarker; Heart failure.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700602
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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Mitochondrial and microsatellite genetic differentiation of the flat oyster Ostrea edulis along the european coasts, and comparison with allozyme data ArchiMer
Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes.
Tipo: Text Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf
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Mitochondrial and nuclear DNA phylogeography of two cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf
Registros recuperados: 97
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